Semester (Jan-May 2026)
BT 520 Applied Biology and Bio-engineering Lab (0-0-6-6) (M.Tech-BT)
Aseptic microbial culture techniques, propagation of host
E.coli strain and storage; Preparation of competent
E. coli for transformation; Bioinformatics tools for
sequence analysis, design of primers and identification of
restriction sites within the transgene. Transformation of E.
coli with recombinant plasmid, carrying the transgene;
Isolation of recombinant plasmid and confirmation of clones by
restriction analysis; Expression of the recombinant protein in
bioreactor (fed batch mode): Determination of specific growth
rate (in terms of Biomass) and expression profile of the
recombinant protein (by SDS PAGE); Agrobacterium-mediated
plant transformation, direct DNA delivery and analysis of
transgene using reporter gene assay.
HT511 Stem Cells and Therapeutics (M.Tech)
Fundamentals of stem
cells: Origin and different types of stem cells: embryonic,
adult, and induced pluripotent stem cells; Genetic reprogramming
and application: Gene expression, stem cell signalling and
transcription factors, differentiation, application in
personalized medicine; Genome Editing: History of genetic
engineering, Gene Editing - gene targeting, CRISPR technology,
multiplex automated genomic engineering, Engineered nuclease:
meganuclease and zinc finger nuclease; Synthetic Biology:
Understanding and creating metabolic and regulatory pathways;
DNA synthesis technology, microfluidics; computational protein
design; chimeric protein; modified/unnatural nucleotides;
Biomedical application of stem cells: Drug screening,
toxicology, disease modelling, cell therapy. iPS application for
treatment of disease conditions: diabetes, leukaemia, and
neurological disorders; Modeling and analytics for stem cell
biology: Clustering, PCA, and enrichment analysis. Machine
learning for classification. Data visualization methods.
Artificial intelligence algorithms to assist iPSC
identification. Modeling of single-cell data; Current trends in
stem cells: Stem cell application in tissue engineering &
biomaterials; Precision medicine, treatment of genetic disorders
and autoimmune; legal guidelines governing embryonic stem cells
research.
Courses Taught
Undergraduate courses (B.Tech)
BT 101 Modern Biology
BT 202M Molecular Biotechnology
BT 204 Genetics
BT 208 Molecular Biology and Genetic
Engineering
BT 303 Immunology
BT 308 Animal Cell Biotechnology
BT 308 Bioengineering
BT 301M Cellular Biotechnology
BT 312 Analytical Biotechnology Lab
Postgraduate
courses (B.Tech, M. Tech and Ph.D)
BT 503 Advanced Genetic Engineering
BT 540 Applied Bioengineering Lab
BT 605 Gene Therapy
BT 632 Stem
Cells, Cancer & Therapy
BT 636 Tissue Engineering and
Stem Cells
HT 502 Basic Physiology for Clinical Immersion
HT501 Introduction to Cellular Processes
HT 511 Stem Cells and Therapeutics
Lab courses
BT540 Applied Bioengineering Lab
Elective courses developed
BT 632 Stem
Cells, Cancer & Therapy
BT 637 Genome Editing and
Engineering
