Unlocking stem cell biology for next-generation regenerative therapies

Laboratory for Stem Cell Engineering and Regenerative Medicine

We develop safe non-integrative strategies for iPSC generation, reprogramming mechanisms, genome editing, and differentiation into functional cell types - including cardiomyocytes, β-cells, and neutrophils - while uncovering transcriptional, epigenetic, and heterogeneity features of stem cells.

Department of Biosciences & Bioengineering (BSBE) · Indian Institute of Technology Guwahati · Guwahati, Assam 781039
SCERM Lab Research Image 1 SCERM Lab Research Image 2 SCERM Lab Research Image 3
29+
Lab Publications
48+
Collaboration Papers
10+
Years of Research
2
Major Grants (SERB/IITG)
1
Deposited iPSC Line
Research Focus Areas

Our interdisciplinary programme spans stem cell biology, protein biochemistry, epigenetics, and translational medicine - all in pursuit of safer, more effective cell reprogramming and regenerative strategies.

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iPSC Generation & Reprogramming

Developing safe, non-integrative strategies to generate induced pluripotent stem cells (iPSCs) from somatic cells, with a focus on protein transduction-based approaches.

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Recombinant Transcription Factors

Producing cell-permeant, biologically active human transcription factors (OCT4, SOX2, NANOG, GATA4, MEF2C, TBX5, HAND2, PDX1 and more) from E. coli for protein transduction.

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Epigenetics & Pluripotency

Investigating epigenetic regulators - including UTF1, NANOG, and chromatin remodellers - that govern stem cell self-renewal, gene expression, and differentiation.

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Directed Differentiation

Differentiating iPSCs into functional cardiomyocytes, β-cells, and neutrophils using defined chemical and molecular protocols for disease modelling and therapy.

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Genome Editing

Applying CRISPR-Cas9 and other genome editing tools in pluripotent stem cells to study gene function and correct disease-causing mutations.

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Stem Cell Heterogeneity

Uncovering transcriptional and epigenetic heterogeneity within stem cell populations using single-cell approaches to better understand reprogramming dynamics.

Recent Highlights
Publication · 2025
UTF1 Expression is Important for the Generation and Maintenance of Human iPSCs
Raina K et al. - Stem Cell Reviews and Reports (IF: 4.2)
iPSC Line · 2023
iPSC line IITGi001-A generated from adult human primary dermal fibroblasts
Khyati et al. - Stem Cell Research (Elsevier)
Grant · 2016–2019
SERB Early Career Research Award (ECRA), DST Govt. of India
Science and Engineering Research Board (SERB)
Dr. Rajkumar P. Thummer

Dr. Rajkumar P. Thummer

Assistant Professor · Department of Biosciences & Bioengineering, IIT Guwahati (July 2015 – Present)

Dr. Rajkumar P. Thummer leads the Laboratory for Stem Cell Engineering and Regenerative Medicine (SCERM Lab) at IIT Guwahati. His research focuses on developing safe, non-integrative strategies for iPSC generation, reprogramming mechanisms, genome editing, and differentiation into functional cell types such as cardiomyocytes, β-cells, and neutrophils. The lab also investigates transcriptional, epigenetic, and heterogeneity features of pluripotent stem cells.

A key focus of the lab is producing biologically active, cell-permeant recombinant human transcription factors from E. coli - including OCT4, SOX2, NANOG, GATA4, MEF2C, TBX5, HAND2, PDX1, UTF1, and others - as tools for protein transduction-based reprogramming. Dr. Thummer played a key role in establishing the Human STEMCCA Cre-Excisable Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit for Merck-Millipore.

📧 rthu@iitg.ac.in  ·  📞 +91-361-258-3208 (Office)  ·  🏛 'O' Block, Office #007 (Ground Floor), BSBE, IIT Guwahati
ResearchGate
Education & Training
07/2015–Present

Assistant Professor

SCERM Lab, BSBE, IIT Guwahati, Assam, India

07/2011–12/2014

Postdoctoral Researcher

Stem Cell Engineering Group, Institute of Reconstructive Neurobiology (RNB), LIFE & BRAIN Center, University of Bonn, Germany

12/2005–12/2010

PhD – Stem Cell Biology

Groningen Biomolecular Sciences & Biotechnology Institute (GBB) / UMCG, University of Groningen, The Netherlands
Thesis: Characterization and role of UTF1 in embryonic stem and carcinoma cells
Supervisor: Dr. Bart Eggen · Ubbo-Emmius PhD Scholarship (2005–2010)

10/2003–09/2004

MSc Biotechnology (Merit)

University of Essex, Colchester, UK

09/1998–04/2002

B.Pharm (First Class)

Anand Pharmacy College, Sardar Patel University, Gujarat, India

Honours & Grants
2016–2019

SERB Early Career Research Award (ECRA)

Science & Engineering Research Board (SERB), DST, Govt. of India

2016–2018

Start-Up Grant

Indian Institute of Technology Guwahati (IITG)

2010

Second Best Oral Presentation Award

"UTF1 regulates ES cell differentiation, chromatin organization and gene expression" - 18th GBB Symposium, Groningen, The Netherlands

2005–2010

Ubbo-Emmius PhD Scholarship

University of Groningen, The Netherlands

01

Non-Integrative iPSC Generation

A primary focus of the lab is developing safe, footprint-free methods to reprogram somatic cells into induced pluripotent stem cells (iPSCs). We use protein transduction of cell-permeant recombinant transcription factors - such as OCT4, SOX2, KLF4, and c-MYC - produced from E. coli, bypassing the risks of viral vector integration. The lab has successfully generated and characterized the iPSC line IITGi001-A from adult human primary dermal fibroblasts (deposited in Stem Cell Research, 2023).

iPSCProtein transductionNon-integrativeFibroblast reprogrammingOCT4 / SOX2 / KLF4
02

Recombinant Human Transcription Factors from E. coli

We have established a robust pipeline for producing biologically active, codon-optimised, cell-permeant versions of pluripotency and cardiac/pancreatic transcription factors from E. coli. To date, the lab has successfully expressed and purified: OCT4, SOX2, NANOG, GLIS1, UTF1, GATA4, MEF2C, MESP1, HAND2, TBX5, PDX1, PAX4, MAFA, NGN3, ETS2, and others. These proteins retain their biological activity and are used for transduction-based cell reprogramming studies.

Recombinant proteinsE. coli expressionCodon optimisationNANOG / OCT4 / SOX2GATA4 / MEF2C / TBX5PDX1 / PAX4
03

Epigenetics, UTF1, and Pluripotency Maintenance

UTF1 (Undifferentiated Embryonic Cell Transcription Factor 1) is a chromatin-associated protein expressed in pluripotent cells. The lab has studied UTF1's roles in ES cell differentiation, chromatin organisation, gene expression, and senescence. Recent work (2025) demonstrates that UTF1 expression is critical for both the generation and maintenance of human iPSCs. We also investigate Nanog's role in suppressing senescence via downregulation of p27KIP1.

UTF1NANOGEpigeneticsChromatinSenescencePluripotency
04

Directed Differentiation into Functional Cell Types

Using iPSCs and other stem cell sources, we develop differentiation protocols to generate clinically relevant cell types. Current projects target cardiomyocytes (for cardiac repair), pancreatic β-cells (for diabetes modelling), and neutrophils (for immune studies). We also collaborate on cancer biology, exploring Wnt/β-catenin pathway inhibition in triple-negative breast cancer via organotrisulfide compounds.

Cardiomyocytesβ-cellsNeutrophilsWnt/β-cateninDisease modelling
05

Interdisciplinary Collaborations

The SCERM Lab actively collaborates with groups across IIT Guwahati and beyond, resulting in joint publications in areas including antimicrobial peptides (AMPs), digital holographic microscopy for cell classification, stimuli-responsive prodrug systems, peroxisome biology, and nanomaterials for magnetic hyperthermia. These collaborations enrich our research with diverse biotechnological and biomedical perspectives.

Antimicrobial peptidesDigital holographyProdrugsPeroxisome biologyNanomaterials
Principal Investigator
Dr. Rajkumar P. Thummer

Dr. Rajkumar P. Thummer

Associate Professor · Department of Biosciences & Bioengineering, IIT Guwahati

Research Interests: Non-integrative somatic reprogramming, footprint-free transcription factor production, human induced pluripotent stem cell line banking, genome editing, and functional directed differentiation targeting diabetes and cardiovascular repair networks.

📧 Email: rthu@iitg.ac.in  •  🏛 Office: 'O' Block, Ground Floor, Room #007, IITG
Current PhD Scholars (Direct Supervision)
Pradeep Kumar S

Pradeep Kumar Sundaravadivelu

PhD Scholar (PMRF)

Joined: January, 2021

Research Project: Elucidating the role of human ZSCAN4 in the generation of induced pluripotent stem cells

Email: p.sundaravadivelu@iitg.ac.in

ResearchGate Profile
Atreyee Borthakur

Atreyee Borthakur

PhD Scholar (PMRF)
IIT Guwahati

Joined: January, 2022

Research Project: Generation of integration-free functional cardiomyocytes by direct reprogramming strategy for prospective cell therapeutic applications

Email: atreyee@iitg.ac.in

ResearchGate Profile
Naveen Raj M

Naveen Raj M

PhD Scholar
IIT Guwahati

Joined: August, 2021

Research Project: Direct reprogramming of human fibroblasts into pancreatic beta-cells using pancreatic-specific transcription factors

Email: naveenraj@iitg.ac.in

ResearchGate Profile
Harsh Vikram Singh

Harsh Vikram Singh

PhD Scholar
IIT Guwahati

Joined: July, 2023

Research Project: iPSCs-derived neutrophils expressing chimeric antigenic receptor for targeted breast cancer therapy

Email: harsh.vsingh@iitg.ac.in

ResearchGate Profile
Ayushi

Ayushi

PhD Scholar
IIT Guwahati

Joined: July, 2025

Research Project: Unraveling the functional role of ZSCAN4 in the maintenance of pluripotency

Email: ayushi@iitg.ac.in

ResearchGate Profile
PhD Scholars (Co-Supervised & Partner Institutes)
👩‍🔬

Shinchini Barman

Co-Supervised
Host: IIT Guwahati (BSBE)
Primary Co-supervisor: Prof. S. S. Bag

Joined: January, 2019

Research Project: Enzymatic incorporation of unnatural nucleobases into DNA and incorporation of unnatural amino acids into proteins

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MD Badirujjaman

Co-Supervised
Host: IIT Guwahati (BSBE)
Primary Co-supervisor: Dr. K. P. Bhabak

Joined: July, 2021

Research Project: Enhancement of the chemotherapeutic potential of anti-cancer drugs: targeted and adjuvant delivery of anti-cancer drugs with antioxidants

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Mehak

Co-Supervised
Host: IIT Guwahati (BSBE)
Primary Co-supervisor: Dr. L. M. Pandey

Joined: August, 2021

Research Project: Magnetic nanoformulations for hyperthermia in cancer treatment

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Hirak Jyoti Bhuyan

Co-Supervised
Host: IIT Guwahati (BSBE)
Primary Co-supervisor: Dr. K. P. Bhabak

Joined: July, 2023

Research Project: Stimuli responsive turn on fluorogenic delivery of anticancer drugs

👩‍🔬

Harini Devi M

Co-Supervised
Host: IIT Guwahati (BSBE)
Primary Co-supervisor: Dr. K. P. Bhabak

Joined: July, 2023

Research Project: Chemical modification of DNA-targeting anti-cancer drugs for enhanced therapeutic efficacy

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Shreejata Ghosh

Co-Supervised
Host: IIT Guwahati (BSBE)
Primary Co-supervisor: Dr. K. P. Bhabak

Joined: July, 2023

Research Project: Understanding the role of small-molecule enzyme inhibitors as anti-cancer agents

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Priyanka Elangbam

Co-Supervised
Host: IIT Guwahati (BSBE)
Primary Co-supervisor: Dr. Kapish Gupta

Joined: January, 2025

Research Project: Development of urine-derived, mechanically reprogrammed hepatocytes with functional bile canaliculi

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Ritama Mukherjee

Co-Supervised
Host: IIT Guwahati (BSBE)
Primary Co-supervisor: Prof. Debasis Manna

Joined: July, 2025

Research Project: Development of modified biopolymers with antibacterial activities

Current M.Tech Students
Subramanian V

Subramanian V

M.Tech Scholar
IIT Guwahati (BSBE)

Timeline: Expected Completion 2026

Thesis Title: Stabilization of purified cardiac-specific recombinant proteins to enable protein-based direct cardiac reprogramming

Status: Active Research Framework

Dhaya Shankaran P

Dhaya Shankaran P

M.Tech Scholar
IIT Guwahati (BSBE)

Timeline: Expected Completion 2026

Thesis Title: Differentiation of iPSCs into pancreatic beta-like cells using pancreatic specific transcription factors

Status: Active Research Framework

Karthikeyan Kandhan

Karthikeyan Kandhan

M.Tech Scholar
IIT Guwahati (BSBE)

Timeline: Expected Completion 2026

Thesis Title: Comparative in silico evaluation of scFv vs nanobody constructs targeting HER2 domain II

Status: Active Research Framework

Research Interns & Active Project Staff
Asmita Karmakar

Asmita Karmakar

Junior Research Fellow (JRF)
M.Sc. Molecular & Human Genetics
Banaras Hindu University

Joined: August, 2023

Project Assignment: Generation of integration-free human induced pluripotent stem cells using a novel gene delivery vector

Email ID: asmita.k@rnd.iitg.ac.in

Lab Alumni - Completed PhD Degrees
Khyati Raina

Dr. Khyati Raina

PhD Alumna (Graduated 2024)
M.Sc. Panjab University

Registration Period: 2018 – 2024

PhD Thesis Title: Investigating the role of human UTF1 in self renewal, differentiation and reprogramming using a CRISPR/Cas toolbox

Publications: Published high impact studies in Stem Cell Reviews and Reports (2021, 2025) and Stem Cell Research (2023).

H Krishna Kumar

Dr. Krishna Kumar Haridhasapavalan

PhD Alumnus (Graduated 2022)
Current: Postdoctoral Fellow, Univ. of Pennsylvania, USA

Registration Period: 2016 – 2022

PhD Thesis Title: Establishment of a bioactive recombinant protein toolbox for the prospective generation of integration-free cardiomyocytes and other biological applications

View Core Achievements

Awarded the national AWSAR Award 2021 by DST, Govt. of India for popular science story writing. Prominent primary author across Scientific Reports, Current Research in Biotechnology, and Applied Microbiology and Biotechnology.

ResearchGate Profile
Chandrima Dey

Dr. Chandrima Dey

PhD Alumna (Graduated 2023)
M.Sc. Vellore Institute of Technology

Registration Period: 2017 – 2023

PhD Thesis Title: Establishment of a bioactive recombinant protein toolbox for the prospective generation of integration-free induced pluripotent stem cells and other biological applications

Highlights: Received training at Kyoto University (CiRA), Japan (2018). Published widely on GLIS1 expression parameters and OCT4 native bacterial factories.

Gloria Narayan

Dr. Gloria Narayan

PhD Alumna (Graduated 2023)
M.Tech National Institute of Technology Rourkela

Registration Period: 2017 – 2023

PhD Thesis Title: Generation of a bioactive recombinant protein toolbox of pancreatic-specific transcription factors from E. coli

Highlights: Developed cell-permeant expression protocols for human PDX1, NGN3, and MAFA factors.

Lab Alumni - Completed M.Tech Degrees
Allan Blessing

Allan Blessing Harison Raj A

M.Tech Alumnus (Completed 2024)
B.Tech Kamraj College

Thesis Title: Production of bioactive recombinant human ZSCAN4 protein from E. coli

ResearchGate Profile
Ronima KR

Ronima KR

M.Tech Alumna (Completed 2023)
B.Tech APJ Abdul Kalam Tech University

Thesis Title: Optimization of expression and purification conditions for human GLIS1 recombinant protein in a bacterial system

Akriti Agrawal

Akriti Agrawal

M.Tech Alumna (Completed 2021)
IIT Guwahati

Thesis Title: Soluble expression, purification, secondary structure determination and in silico analysis of human PAX4 transcription factor

ResearchGate Profile
Tirthankar Sen

Tirthankar Sen

M.Tech Alumnus (Completed 2022)
IIT Guwahati

Thesis Title: CRISPR and iPSCs: Recent developments and future perspectives in neurodegenerative disease modelling, research and therapeutics

Publications: Published reviews in Frontiers in Immunology and Neurotoxicity Research (2022).

Pradeep Kumar S

Pradeep Kumar Sundaravadivelu

M.Tech Alumnus (Completed 2020)
Current: PhD Track, SCERM Lab

Thesis Title: Molecular cloning, expression, purification and characterization of human GATA4, ETS2 and HAND2 transcription factors

Poulomi Adhikari

Poulomi Adhikari

M.Tech Alumna (Completed 2019)
Current Position: PhD Scholar, Academia Sinica, Taiwan

Thesis Title: Cloning stem cell-specific reprogramming factors in a novel non-integrating viral vector

Bitan Saha

Bitan Saha

M.Tech Alumnus (Completed 2018)
Current Position: PhD student, National University of Singapore

Project Period: 06/2017 - 05/2018

Thesis Title: Cloning and expression of codon optimized stem cell-specific human transcription factor Nanog in a bacterial system

Ankit Narula

Ankit Narula

M.Tech Alumnus (Completed 2017)
Current Position: Entrepreneur, New Delhi

Project Period: 06/2016 - 05/2017

Thesis Title: Analysis of stem cell and related patents, patenting laws and clinical trial regulations in India and other Asian countries

Kanish Nareen V

Kanish Nareen V

M.Tech Alumnus (Completed 2025)
IIT Guwahati

Thesis Title: Enhancing the stability of purified cardiac-specific recombinant proteins and optimizing lentiviral-mediated direct cardiac reprogramming

Lab Alumni - Former B.Tech Scholars & Project Staff
Kumar Rishabh

Kumar Rishabh

Former B.Tech Scholar
Completed: May, 2021

Project scope: Insilico analysis of stem cell-specific transcription factors

Email: rishabh18b@iitg.ac.in

Rahul Doshi

Rahul Doshi

Former B.Tech Scholar
Completed: May, 2021

Project scope: Insilico analysis of stem cell-specific transcription factors

Email: rahul@iitg.ac.in

Arnab Ray

Arnab Ray

Former B.Tech Scholar
Completed: May, 2020

Project Title: Heterologous expression, purification, characterization and intra-cellular delivery of Human NANOG

Email: arnab170106013@iitg.ac.in

Nayan Jyoti Das

Nayan Jyoti Das

Former B.Tech Scholar
Completed: May, 2019

Project Title: Expression, purification and characterization of recombinant TBX5

Email: nayannjddas@iitg.ac.in

Ashutosh Bhavsar

Ashutosh Bhavsar

Former B.Tech Scholar
Completed: July, 2018

Project Title: Expression analysis of cell reprogramming transcription factor human GATA-3

Email: a.bhavsar@iitg.ac.in

Sankalita Paul

Sankalita Paul

Former Junior Research Fellow (JRF)
Completed: Feb, 2018 Frame

Project scope: Generation of transgene-free human induced pluripotent stem cells using non-genetic approaches

Current Position: PhD Student; IISER Mohali, Chandigarh

Manash P Borgohain

Manash P Borgohain

Former M.S. (Pharm) Scholar
Host: NIPER Guwahati Framework

Specialization: Pharmacology & Toxicology

Key Publications: Co-authored papers on non-integrative delivery and bacterial recombinant factors in Stem Cell Reviews and Gene.

2026 Publications
Developmental stage-dependent cellular plasticity governs β-cell reprogramming efficiency in human fibroblasts Corresponding Author IF: 3.1 · Q2
Raj N, Panneerselvam DS, Thummer RP
Biochemical and Biophysical Research Communications, 754, 154188. https://doi.org/10.1016/j.bbrc.2026.154188
Derivation and characterization of the iPSC line IITGi001-B from primary human adult dermal fibroblasts Corresponding Author IF: 4.1 · Q2
Sundaravadivelu PK, Raina K, Borthakur A, Mahto P, Shetty D, Dhamne C, Kaveeshwar V, Martin CA, Radhakrishnan S, Rela M, Thummer RP
Stem Cell Research, 103, 103931. https://doi.org/10.1016/j.scr.2026.103931
Surface functionalization of iron oxide nanoparticles with polymers and small molecules: Synthesis, characterization, and evaluation of magnetic hyperthermia performance IF: 4.4 · Q2
Mehak, Patton MM, Borah JP, Thummer RP, Pandey LM
Macromolecular Bioscience, 26(3), e00632. https://doi.org/10.1002/mabi.202500632
2025 Publications
UTF1 expression is important for the generation and maintenance of human iPSCs Corresponding Author IF: 4.2 · Q2
Raina K, Modak K, Premkumar C, Joshi G, Palani D, Nandy K, Sivamani Y, Velayudhan SR, Thummer RP
Stem Cell Reviews and Reports, 21, 859-871. https://doi.org/10.1007/s12015-024-10836-x
Leveraging cardiac reprogramming with CRISPRa-SAM technology Corresponding Author IF: 6.1 · Q1
Borthakur A, Venugopal KN, Thummer RP
Molecular Therapy Nucleic Acids, 36, 102532. https://doi.org/10.1016/j.omtn2025.102532
Impact of the arrangement modes of the antimicrobial motifs WWR-7 and its analogs in CAMPs on their therapeutic properties IF: 3.3 · Q2
Yadav M, Singh HV, Basu S, Thummer RP, Chatterjee S
Chemistry - An Asian Journal, 21, e70330. https://doi.org/10.1002/asia.70330
Lateral shearing common-path digital holographic microscopy with iterative reconstruction IF: 1.7 · Q2
Chaudhari H, Das A, Singh HV, Ghosh A, Kulkarni R, Bhuyan MK, Thummer RP
Applied Optics, 64, 6285-6291. http://dx.doi.org/10.1364/AO.564574
Serum-stable; cationic; alpha-helical AMPs to combat infections of ESKAPE pathogens and C. albicans IF: 4.7 · Q1
Sarkar T; Rajalakshmi VS; Ronima KR; Thummer RP; Chatterjee S
ACS Applied Bio Materials, 8, 3941-3957. https://doi.org/10.1021/acsabm.5c00126
Salt-tolerant; protease-stable and non-resistance developing cationic AMPs for combatting planktonic MRSA and its biofilms IF: 4.0 · Q1
Yadav M; Singh T; Singh HV; Thummer RP; Chatterjee S
ACS Medicinal Chemistry Letters, 16, 1089-1097. https://doi.org/10.1021/acsmedchemlett.5c00121
Cumulative learning & technology based segmentation aided cell mixtures classification in digital holographic microscopy IF: 5.0 · Q1
Chaudhari H; Sundaravadivelu PK; Kulkarni R; Bhuyan MK; Thummer RP
Optics Laser Technology, 181, 112029. https://doi.org/10.1016/j.optlastec.2024.112029
2024 Publications
Esterase-responsive self-immolative prodrugs for the sustained delivery of the anticancer drug 5-Fluorouracil with turn-on fluorescence Corresponding Author IF: 3.3 · Q2
Badirujjaman M; Thummer RP; Bhabak KP
Chemistry - An Asian Journal, 20, e202400846. https://doi.org/10.1002/asia.202400846
De novo design of Tryptophan containing broad-spectrum cationic antimicrobial octapeptides IF: 3.4 · Q1
Sarkar T; Vignesh SR; Sundaravadivelu PK; Thummer RP; Satpati P; Chatterjee S
ChemMedChem, 20, e202400566. https://doi.org/10.1002/cmdc.202400566
Development of protease resistant and non-cytotoxic Jelleine analogs with enhanced broad spectrum antimicrobial efficacy IF: 2.5 · Q1
Sarkar T; Vignesh SR; Sehgal T; Ronima KR; Thummer RP; Satpati P; Chatterjee S
Biochimica et Biophysica Acta - Biomembranes, 1866, 184336. https://doi.org/10.1016/j.bbamem.2024.184336
Mechanism of protease resistance of D-amino acid residue containing cationic antimicrobial heptapeptides IF: 3.8 · Q1
Sarkar T; Ghosh S; Sundaravadivelu PK; Pandit G; Debnath S; Thummer RP; Satpati P; Chatterjee S
ACS Infectious Diseases, 10, 562-581. https://doi.org/10.1021/acsinfecdis.3c00491
Tuning of hydrophobic-hydrophilic balance for the development of a salt-tolerant and protease-resistant lipopeptide AMP IF: 2.5 · Q2
Chetia M; Sarkar T; Yadav M; Dey C; Sundaravadivelu PK; Thummer RP; Chatterjee S
New Journal of Chemistry, 48, 2764-2777. https://doi.org/10.1039/d3nj04815b
Dimensionality reduction technique based phase aberration compensation and spurious fringe removal in off-axis digital holographic microscopy IF: 3.7 · Q1
Chaudhari H; Kulkarni R; Sundaravadivelu PK; Thummer RP; Bhuyan MK
Optics and Lasers in Engineering, 172, 107853. https://doi.org/10.1016/j.optlaseng.2023.107853
Digital hologram reconstruction algorithm based on fractional fourier transform in non-telecentric digital holographic microscopy IF: 3.3 · Q1
Chaudhari H; Kulkarni R; Sundaravadivelu PK; Thummer RP; Bhuyan MK
Optics Letters, 49, 182-185. https://doi.org/10.1364/OL.504723
2023 Publications
Biological activity of recombinant human PDX1 protein produced from Escherichia coli Corresponding Author IF: 2.8 · Q2
Narayan G; Sen P; Nagotu S; Thummer RP
Journal of Biochemical and Molecular Toxicology, 37, e23511. https://doi.org/10.1002/jbt.23511
Production of bioactive human PAX4 protein from E. coli. Corresponding Author IF: 1.4 · Q3
Narayan G; Agrawal A; Sen P; Nagotu S; Thummer RP
The Protein Journal, 42, 766-777. https://doi.org/10.1007/s10930-023-10143-3
Generation and characterization of induced Pluripotent Stem Cell line IITGi001-A derived from adult human primary dermal fibroblasts Corresponding Author IF: 0.7 · Q3
Raina K; Joshi G; Modak K; Premkumar C; Priyanka S; Rajesh P; Velayudhan SR; Thummer RP
Stem Cell Research, 71, 103159. https://doi.org/10.1016/j.scr.2023.103159
Surface modified iron-oxide based engineered nanomaterials for hyperthermia therapy of cancer cells IF: 1.9 · Q2
Mehak; Thummer RP; Pandey LM
Biotechnology and Genetic Engineering Reviews, 39, 1187-1233. https://doi.org/10.1080/02648725.2023.2169370
2022 Publications
Characterization of the multiple domains of Pex30 involved in subcellular localization of the protein and regulation of peroxisome number IF: 2.5 · Q2
Deori NM; Infant T; Thummer RP; Nagotu S
Cell Biochemistry and Biophysics, 81, 39-47. https://doi.org/10.1007/s12013-022-01122-z
Gelatin-PVA-Electronic AgN/As triad composite as wound healing hydrogel with wounded skin surface protective efficiency Preprint Portfolio
Bag SS; Bora A; Golder A; Raina K; Haridhasapavalan KK; Thummer RP
SSRN Electronic Journal, N/A. http://dx.doi.org/10.2139/ssrn.4219683
Generation of a recombinant version of a biologically active cell-permeant human HAND2 transcription factor from E. coli Corresponding Author IF: 3.9 · Q1
Haridhasapavalan KK; Sundaravadivelu PK; Joshi N; Das NJ; Mohapatra A; Voorkara U; Kaveeshwar V; Thummer RP
Scientific Reports, 12, 16129. https://doi.org/10.1038/s41598-022-19745-w
CRISPR and iPSCs: Recent developments and future perspectives in neurodegenerative disease modelling; research and therapeutics Corresponding Author IF: 3.3 · Q2
Sen T; Thummer RP
Neurotoxicity Research, 40, 1597-1623. https://doi.org/10.1007/s12640-022-00564-w
Targeting Wnt/beta-catenin signaling pathway in triple-negative breast cancer by benzylic organotrisulfides Corresponding Author IF: 8.2 · Q1
Bhattacherjee D; Raina K; Mandal TK; Thummer RP; Bhabak KP
Free Radical Biology and Medicine, 191, 82-96. https://doi.org/10.1016/j.freeradbiomed.2022.08.029
The impact of human microbiotas in hematopoietic stem cell and organ transplantation Corresponding Author IF: 5.9 · Q1
Sen T; Thummer RP
Frontiers in Immunology, 13, 932228. https://doi.org/10.3389/fimmu.2022.932228
A comprehensive review on the role of ZSCAN4 in embryonic development; stem cells; and cancer Corresponding Author IF: 4.2 · Q2
Thool M; Sundaravadivelu PK; Sudhagar S; Thummer RP
Stem Cell Reviews and Reports, 18, 2740–2756. https://doi.org/10.1007/s12015-022-10412-1
Chemical Stimuli-responsive prodrug of non-steroidal anti-inflammatory drug diclofenac: self-immolative drug release with turn-on near-infrared fluorescence IF: 4.2 · Q1
Sufian A; Bhattacherjee D; Barman P; Srivastava A; Thummer RP; Bhabak KP
Chemical Communications, 58, 7833-7836. https://doi.org/10.1039/d2cc02132c
Benzimidazole-based ionic and non-ionic organoselenium compounds: Innovative synthetic strategies; structural characterizations and preliminary anti-proliferative activities Corresponding Author IF: 2.5 · Q2
Banerjee K; Bhattacherjee D; Raina K: Thummer RP; Bhabak KP
New Journal of Chemistry, 46, 11910-11926. https://doi.org/10.1039/d2nj01322c
Pex30 undergoes phosphorylation and regulates peroxisome number in Saccharomyces cerevisiae IF: 2.1 · Q2
Deori NM; Infant T; Sundaravadivelu PK; Thummer RP; Nagotu S
Molecular Genetics and Genomics, 297, 573-590. https://doi.org/10.1007/s00438-022-01872-8
Generation of a transducible version of a bioactive recombinant human TBX5 transcription factor from E. coli Corresponding Author IF: 4.0 · Q2
Haridhasapavalan KK; Das NJ; Thummer RP
Current Research in Biotechnology, 4, 66-77. https://doi.org/10.1016/j.crbiot.2022.01.004
2021 Publications
Protein production and purification of a codon-optimized human NGN3 transcription factor from E. coli Corresponding Author IF: 1.4 · Q3
Narayan G; Agrawal A; Joshi N; Gogoi R; Nagotu S; Thummer RP
The Protein Journal, 40, 891-906. https://doi.org/10.1007/s10930-021-10020-x
Identification of optimal expression parameters and purification of a codon-optimized human GLIS1 transcription factor from Escherichia coli Corresponding Author IF: 2.5 · Q2
Dey C; Venkatesan V; Thummer RP
Molecular Biotechnology, 64, 42-56. https://doi.org/10.1007/s12033-021-00390-z
An overview on promising somatic cell sources utilized for the efficient generation of induced pluripotent stem cells Corresponding Author IF: 4.2 · Q2
Ray A; Joshi JM; Sundaravadivelu PK; Raina K; Lenka N; Kaveeshwar V; Thummer RP
Stem Cell Reviews and Reports, 17, 1954-1974. https://doi.org/10.1007/s12015-021-10200-3
Generation of biologically active recombinant human OCT4 protein from E. coli Corresponding Author IF: 2.9 · Q1
Dey C; Thool M; Bhattacharyya S; Sudhagar S; Thummer RP
Soluble expression; purification; and secondary structure determination of human MESP1 transcription factor Corresponding Author IF: 4.3 · Q1
Haridhasapavalan KK; Ranjan SH; Bhattacharyya S; Thummer RP
Applied Microbiology and Biotechnology, 105, 2363-2376. https://doi.org/10.1007/s00253-021-11194-1
Generation of a recombinant stem cell-specific human SOX2 protein from Escherichia coli under native conditions Corresponding Author IF: 2.5 · Q2
Thool M; Dey C; Bhattacharyya S; Sudhagar S; Thummer RP
Molecular Biotechnology, 63, 327-338. https://doi.org/10.1007/s12033-021-00305-y
An insight into the role of UTF1 in development; stem cells; and cancer Corresponding Author IF: 4.2 · Q2
Raina K; Dey C; Thool M; Sudhagar S; Thummer RP
Stem Cell Reviews and Reports, 17, 1280-1293. https://doi.org/10.1007/s12015-021-10127-9
Generation of cell-permeant recombinant human transcription factor GATA4 from E. coli Corresponding Author IF: 3.6 · Q2
Narayan G; Sundaravadivelu PK; Agrawal A; Gogoi R; Nagotu S; Thummer RP
Bioprocess and Biosystems Engineering, 44, 1131-1146. https://doi.org/10.1007/s00449-021-02516-8
2016 – 2020 Historical Archives
Soluble expression; purification; and secondary structure determination of human PDX1 transcription factor Corresponding Author IF: 1.2 · Q3
Haridhasapavalan KK; Sundaravadivelu PK; Thummer RP
Protein Expression and Purification, 180, 105807. https://doi.org/10.1016/j.pep.2020.105807
Codon optimization; molecular cloning; expression; purification; and secondary structure determination of human ETS2 transcription factor Corresponding Author IF: 2.5 · Q2
Haridhasapavalan KK; Sundaravadivelu PK; Bhattacharyya S; Ranjan SH; Raina K; Thummer RP
Molecular Biotechnology, 62, 485-494. https://doi.org/10.1007/s12033-020-00266-8
An insight into reprogramming barriers to iPSC generation Corresponding Author IF: 4.2 · Q2
Haridhasapavalan KK; Raina K; Dey C; Adhikari P: Thummer RP
Stem Cell Reviews and Reports, 16, 56-81. https://doi.org/10.1007/s12015-019-09931-1
A stimuli-responsive anticancer drug delivery system with inherent antibacterial activities IF: 4.2 · Q1
Dey S; Patel A; Raina K; Pradhan N; Biswas O; Thummer RP; Manna D
Chemical Communications, 56, 1661-1664. https://doi.org/10.1039/c9cc08834b
An insight into DNAfree-reprogramming approaches to generate integration-free induced pluripotent stem cells Corresponding Author IF: 4.2 · Q2
Borgohain MP; Haridhasapavalan KK; Dey C; Adhikari P: Thummer RP
Stem Cell Reviews and Reports, 15, 286-313. https://doi.org/10.1007/s12015-018-9861-6
An insight into non-integrative gene delivery approaches to generate transgene-free induced pluripotent stem cells Corresponding Author IF: 2.4 · Q2
Haridhasapavalan KK; Borgohain MP; Dey C; Saha B; Narayan G; Kumar S; Thummer RP
Prospective applications of induced pluripotent stem cells in military medicine Corresponding Author IF: 0.73 · Q3
Saha B; Haridhasapavalan KK; Borgohain MP; Thummer RP
Medical Journal Armed Forces India, 74, 313-320. https://doi.org/10.1016/j.mjafi.2018.03.005
iPS cell generation: current and future challenges Corresponding Author
Saha B; Borgohain MP; Dey C; Thummer RP
Annals of Stem Cell Research and Therapy, 1, 1007.
Cell-penetrating peptides as a tool to deliver biologically active recombinant proteins to generate transgene-free induced pluripotent stem cells Corresponding Author
Dey C; Narayan G; Haridhasapavalan KK; Borgohain MP; Lenka N; Thummer RP
Studies on Stem Cells Research and Therapy, 3, 006-015. https://doi.org/10.17352/sscrt.000011
Nanog induces suppression of senescence through downregulation of p27KIP1 expression Corresponding Author IF: 3.6 · Q2
Munst B; Thier MC; Winnemöller D; Helfen M; Thummer RP; Edenhofer F
Journal of Cell Science, 129, 912-920. https://doi.org/10.1242/jcs.167932
Book Chapters & Conference Proceedings (Scopus Indexed)
Design and validation of CRISPR-Cas9 mediated knockout of ZSCAN4 gene in mammalian cells Corresponding Author
Sundaravadivelu PK; Thummer RP
In: Recent Developments in Biotechnology: Proceedings of Research and Industrial Conclave – Synergy 2025. Springer Nature, 2026.
Generation of cell permeant recombinant ZSCAN4 protein for various biological applications Corresponding Author
Sundaravadivelu PK; Augustine ABR; Thummer RP
In: Recent Developments in Biotechnology: Proceedings of Research and Industrial Conclave – Synergy 2025. Springer Nature, 2026.
Small molecules and epigenetic modifiers in facilitating pancreatic beta-cell formation: A comprehensive insight
Raj N; Karmakar A; Narayan G; Thummer RP
Cell Biology and Translational Medicine; Volume 26 (Springer Cham), 2025, pp. 77-102.
Genes as genome stabilizers in pluripotent stem cells
Karmakar A; Augustine ABHR; Thummer RP
Cell Biology and Translational Medicine; Volume 25 (Springer Cham), 2025, pp. 21-47.
Common path off-axis digital holographic flow cytometry based on lateral shearing using beam splitter
Chaudhari H; Das A; Singh HV; Ghosh A; Kulkarni R; Bhuyan MK; Thummer RP
Proceedings of SPIE, Volume 13571, 2025.
An overview of the generation of integration-free induced pluripotent stem cells and their subsequent differentiation into cardiomyocytes and beta-cells
Sundaravadivelu PK; Borthakur A; Agrawal A; Sen T; Thummer RP
Stem Cell Biology: A Regenerative Tissue Perspective (World Scientific Connect), 2025, pp. 95-149.
Machine-learning-based classification of co-cultured cells from the phase images in digital holographic microscopy
Chaudhari H; Kulkarni R; Bhuyan MK; Sundaravadivelu PK; Thummer RP
Proceedings of SPIE, Volume 12996, 2024, pp. 87-96.
Engineered iron-oxide based nanomaterials for magnetic hyperthermia (Innovations; Improvements; Inclusion and Impact)
Mehak; Thummer RP; Pandey LM; Srivatsan TS
Advanced Materials for Emerging Applications (Bentham Science Publisher), 2024, pp. 440-463.
An insight into the role of GLIS1 in embryonic development; iPSC generation and cancer
Ronima KR; Dey C; Thummer RP
Cell Biology and Translational Medicine; Volume 22 (Springer Cham), 2023, pp. 97-113.
An insight into vital genes responsible for beta-cell formation
Narayan G; Ronima KR; Agrawal A; Thummer RP
Cell Biology and Translational Medicine; Volume 21 (Springer Cham), 2023, pp. 1-27.
Direct cardiac reprogramming: Current status and future prospects
Haridhasapavalan KK; Borthakur A; Thummer RP
Cell Biology and Translational Medicine; Volume 20 (Springer Cham), 2023, pp. 1-18.
Generation of recombinant version of a bioactive human MEF2C transcription factor from E. coli
Haridhasapavalan KK; Sundaravadivelu PK; Voorkara U; Kaveeshwar V; Thummer RP
Proceedings of NERC 2022 (Springer Singapore), 2023, pp. 3-27.
Production of a bioactive recombinant human UTF1 protein from E. coli
Dey C; Raina K; Thummer RP
Proceedings of NERC 2022 (Springer Singapore), 2023, pp. 63-83.
Soluble expression and purification of biologically active human NANOG from Escherichia coli
Thool M; Sudhagar S; Thummer RP
Proceedings of NERC 2022 (Springer Singapore), 2023, pp. 99-118.
Heterologous expression; purification; and biological activity determination of codon-optimized human MAFA transcription factor from E. coli
Narayan G; Nagotu S; Thummer RP
Proceedings of NERC 2022 (Springer Singapore), 2023, pp. 145-158.
Induced pluripotent stem cell biobanking: A future perspective for regenerative and personalized medicine
Thummer RP; Kaveeshwar V; Nayanabhirama U
Bio Banking for Affordable Healthcare (Manipal University Press), 2023, pp. 188-204.
Direct reprogramming of somatic cells into induced beta-cells: An overview
Narayan G; Ronima KR; Thummer RP
Cell Biology and Translational Medicine; Volume 19 (Springer Cham), 2022, pp. 171-189.
Auxiliary pluripotency-associated genes and their contributions in the generation of induced pluripotent stem cells
Dey C; Raina K; Thool M; Adhikari P; Haridhasapavalan KK; Sundaravadivelu PK; et al.
Advances in Stem Cell Biology; Volume 12 (Academic Press), 2022, pp. 29-94.
Tissue-restricted stem cells as starting cell source for efficient generation of pluripotent stem cells: An overview
Sundaravadivelu PK; Raina K; Thool M; Ray A; Joshi JM; Kaveeshwar V; Sudhagar S; Lenka N; Thummer RP
Cell Biology and Translational Medicine; Volume 15 (Springer Cham), 2021, pp. 151–180.
Recent advances in the generation of beta-cells from induced pluripotent stem cells as a potential cure for diabetes mellitus
Agrawal A; Narayan G; Gogoi R; Thummer RP
Cell Biology and Translational Medicine; Volume 14 (Springer Cham), 2021, pp. 1–27.
An overview of reprogramming approaches to derive integration-free induced pluripotent stem cells for prospective biomedical applications
Dey C; Raina K; Haridhasapavalan KK; Thool M; Sundaravadivelu PK; Adhikari P; Gogoi R; Thummer RP
Advances in Stem Cell Biology; Volume 5 (Academic Press), 2021, pp. 231–287.
Maximizing expression and yield of human recombinant proteins from bacterial cell factories for biomedical applications
Borgohain MP; Narayan G; Krishna Kumar H; Dey C; Thummer RP
Advances in Microbial Biotechnology (Apple Academic Press), 2018, pp. 431-468.
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2025
📄 Publication
UTF1 Expression is Important for the Generation and Maintenance of Human iPSCs
Raina K et al. published in Stem Cell Reviews and Reports (IF: 4.2). This work establishes the critical role of UTF1 in both generating and maintaining human induced pluripotent stem cells - a key advance for the lab's non-integrative iPSC programme.
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2025
🤝 Collaboration
Multiple collaboration papers published in 2025
New collaborative work published in Applied Optics (digital holographic microscopy), ACS Applied Bio Materials (antimicrobial peptides), and ACS Med Chem Lett (MRSA combat) - with research groups at IIT Guwahati.
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2023
🧬 iPSC Line Deposited
Human iPSC line IITGi001-A generated and characterised
Khyati R, Joshi G, Modak K et al. published the generation and characterisation of iPSC line IITGi001-A from adult human dermal fibroblasts in Stem Cell Research (Elsevier). This marks the first iPSC line deposited by SCERM Lab.
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2022
📄 Publication - Springer Nature
Cell-permeant recombinant human HAND2 transcription factor produced from E. coli
Haridhasapavalan KK, Sundaravadivelu PK et al. published in Scientific Reports (IF: 4.996). The paper demonstrates generation of a biologically active, cell-permeant HAND2 protein - a cardiac reprogramming factor.
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2016
🏆 Grant Award
SERB Early Career Research Award (ECRA) awarded to Dr. Thummer
Dr. Rajkumar P. Thummer was selected for the Young Scientist Award (ECRA) by the Science and Engineering Research Board (SERB), DST, Govt. of India. The project ran from 2016 to 2019.
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2015
🏛 Lab Founded
SCERM Lab established at IIT Guwahati
Dr. Rajkumar P. Thummer joined IIT Guwahati as Assistant Professor in July 2015 and established the Laboratory for Stem Cell Engineering and Regenerative Medicine (SCERM Lab) in the Department of Biosciences & Bioengineering.

Find Us

📍IIT Guwahati Campus, Assam
Address

Dr. Rajkumar P. Thummer
'O' Block, Office #007 (Ground Floor)
Laboratory for Stem Cell Engineering and Regenerative Medicine (SCERM Lab)
Department of Biosciences & Bioengineering (BSBE)
Indian Institute of Technology Guwahati (IITG)
Amingaon, North Guwahati, Assam – 781039, India

Phone (Office)

+91-361-258-3208

Joining the Lab

Prospective PhD students should apply through the IIT Guwahati PhD admissions portal. For JRF or internship enquiries, email Dr. Thummer with your CV and a brief statement of research interest.

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